GeoMx® RNA Panel for Immuno-Oncology

The GeoMx® Immune Pathways panel is designed for comprehensive profiling of the tumor, tumor microenvironment, and tumor immune status. Profile up to 96 curated RNA targets with spatial resolution from a single tissue section using the GeoMx Digital Spatial Profiler (DSP).

GeoMx® RNA Assay Design

Designed to profile up to 96 targets simultaneously with spatial resolution, the Immune Pathways Core contains 84 targets plus controls designed for broad coverage of the tumor and tumor microenvironment. Up to 10 custom targets with 2 additional controls can be added to select the content most relevant to your research. GeoMx® RNA assays contain in situ hybridization (ISH) probes conjugated to unique DNA indexing-oligonucleotides via a UV-photocleavable linker. After region of interest (ROI) selection on GeoMx® DSP and UV cleavage of the oligonucleotides, each DNA oligonucleotide is recognized by a unique reporter probe that contains a fluorescent barcode. Reporter probes are imaged and counted by the nCounter® Analysis System to provide a direct, digital readout of spatially resolved RNA expression.

Curated Content for Immuno-Oncology

The GeoMx® Immune Pathways panel is designed to profile key aspects of tumor and tumor microenvironment biology.

• Profile the global immune response
• Assess microenvironment immune activity
• Quantify tumor reactivity
• Measure the 18-gene Tumor Inflammation Signature known to be associated with response to PD-1/PD-L1 inhibitor pathway blockade

Accompanying Morphology Marker Kits are available for tissue visualization and ROI selection.

GeoMx® TCR Profiling Add-On

Gene Expression Add-On to HuWTA or CTA

Add the ability to spatially profile expression of different T Cell Receptor (TCR) Variable and Joining regions to your GeoMx® DSP Human Whole Transcriptome Atlas (HuWTA) or Cancer Transcriptome Atlas (CTA) assay. Spatially quantify changes in TCR diversity in response to cancer, infectious disease, autoimmunity, organ rejection, and therapeutic treatment within distinct tissue compartments or cell type populations within tissue sections.

Highlights

• Add an additional dimension to your HuWTA or CTA experiments with the TCR Profiling Add-On
• Collect spatial expression data on 146 Human T Cell Receptor segments, including T Cell Receptor Variable and Joining regions across Alpha, Beta, Gamma, and Delta chains
• Customize with up to 400 additional targets to assay additional T cell biology content, non-coding RNA, synthetic DNA, or exogenous genes
• Overlapping content with the nCounter® TCR Diversity Panel allows for paired sample studies Chain Type Variable Regions, V Joining Regions, J Constant Regions, C Alpha TRAV

The TCR Profiling Add-On assay for GeoMx® DSP contains probes for 146 Variable and Joining segments across all four Human TCR receptor loci Alpha, Beta, Gamma, and Delta plus 50 negative control targets and 6 positive T cell controls. Combined with the GeoMx® HuWTA or CTA assays which contain probes for TCR Constant regions and key markers of T Cell phenotype and activation status, the TCR Profiling Add-On is a powerful tool that enables you to fully understand the adaptive T cell immune response to different diseases and/or therapeutic treatment.

Applications

The GeoMx® TCR Profiling Add-On can used in combination with the HuWTA or the CTA to spatially profile in FFPE or fresh frozen tissue the effect of disease onset/progression and/or treatment response/vaccination for any number of application areas:

• Understanding the effect of CAR-T cells or engineered T cells on the T cell adaptive immune response to cancer
• Monitoring the effect of vaccination or the impact of disease resolution on the T cell response to infectious disease
• Studying the impact of T cell mediated organ rejection in solid organ transplantation
• Using spatial localization of T cell receptor diversity as a potentially predictive or prognostic biomarker for disease progression, severity, or treatment response